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Korean J Physiol Pharmacol 2017 Sep; 21(5): 465-474
Fanhua Kong1,2,5, Runqi Zhang2, Xudong Zhao3, Guanlin Zheng4, Zhou Wang5,*, and Peng Wang2
Resveratrol raises in vitro anticancer effects of paclitaxel in NSCLC cell line A549 through CO X-2 expression
1School of Medicine, Shandong University, Jinan 250000, Shandong, Departments of 2Thoracic Surgery, 3Gynaecology and Obstetrics, Taian City Central Hospital, 4Taian Vocational College of Nursing, Taian 271000, Shandong, 5Department of Thoracic Surgery, Shandong Provincial Hospital affiliated to Shandong University, Jinan 250000, Shandong, China
The aim of this study was to determine the raising anticancer effects of resveratrol (Res) on paclitaxel (PA) in non-small cell lung cancer (NSCLC) cell line A549. The 10 µg/ml of Res had no effect on human fetal lung fibroblast MRC-5 cells or on A549 cancer cells and the 5 or 10 µg/ml of PA also had no effect on MRC-5 normal cells. PA-L (5 µg/ml) and PA-H (10 µg/ml) had the growth inhibitory effects in NSCLC cell line A549, and Res increased these growth inhibitory effects. By flow cytometry experiment, after Res (5 µg/ml)+PA-H (10 µg/ml) treatment, the A549 cells showed the most apoptosic cells compared to other group treatments, and after additional treatment with Res, the apoptosic cells of both two PA concentrations were raised. Res+PA could reduce the mRNA and protein expressions of COX-2, and Res+PA could reduce the COX-2 related genes of VEGF, MMP-1, MMP-2, MMP-9, NF-κB, Bcl-2, Bcl-xL, procollagen I, collagen I, collagen III and CTGF, TNF-α, IL-1β, iNOS and raise the TIMP-1, TIMP-2, TIMP-3, IκB-α, p53, p21, caspase-3, caspase-8, caspase-9, Bax genes compared to the control cells and the PA treated cells. From these results, it can be suggested that Res could raise the anticancer effects of PA in A549 cells, thus Res might be used as a good sensitizing agent for PA.
Keyword : A549, Cancer, Gene, Paclitaxel, Resveratrol